| 摘要: |
| 为了寻求简便、快速的辣椒染色体倍性鉴定方法,以辣椒纯系B19、B23花药培养产生的单倍体植株（n=x=12）及用其种子长成的二倍体植株为试材,对用气孔保卫细胞叶绿体计数法鉴定其倍数性进行了研究。结果表明,单倍体和二倍体辣椒植株气孔保卫细胞叶绿体数平均值的差异极显著,单倍体的叶绿体数在13以下,二倍体的叶绿体数等于或大于13;气孔叶绿体数目随染色体倍数性的增加而增加,用气孔保卫细胞叶绿体数目预测植株倍性的准确率可达92.68%。表明采用气孔保卫细胞叶绿体计数法可以在苗期快速、准确地确定植株的染色体倍性。 |
| 关键词: 辣椒 染色体 气孔保卫细胞叶绿体计数法 倍性鉴定 |
| DOI： |
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| 基金项目:国家“十一五”科技支撑计划项目（2006BAD01A7）；西北农林科技大学植物遗传育种专项(05YZ024-1) |
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| Rapid determination of ploidy level of chromosome in pepper |
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| Abstract: |
| In order to seek a simple and rapid method of determinating the ploidy level of chromosome in pepper,the haploid plant of anthers and diploid plant of seed derived from B19 and B23 of pepper(Capsicum annuum L.) were identified by counting the number of stoma chloroplast in guard cell.The results showed that the discrepancy of the average chloroplast number was extremely prominent.Chloroplast number of the haploid was below 13,and that of the diploid was equal to or more than 13.The number of stomatal chloroplasts increased with the increase of ploidy levels.The reliability of ploidy identification counted by stoma guard cell chloroplast number was higher,with the average accurate rate of 92.68%,showing that measurement of the number of stoma chloroplast in guard cell could be considered as a fast and accurate method to determine haploid and diploid of pepper in the seedling stage. |
| Key words: pepper(Capsicum annuum L.),chloroplast,counting the number of stoma chlorlplast in guard cell,determination of ploidy Level |
