| 摘要: |
| 为了筛选玉米耐盐愈伤组织变异体,选用4个适于玉米组织培养基因型的幼胚为外植体,诱导出胚性愈伤组织。继代5次后,分别用200 mmol/L NaCl和5.5 mmol/L Hyp连续筛选3次,获得耐盐愈伤组织变异体,分别称为rn变异体和rh变异体,以普通继代培养基上培养的愈伤组织为对照。经一代回复培养,分别在200,250和300 mmol/L NaCl胁迫下,分别测定了rn变异体r、h变异体和对照愈伤组织存活率,胚性愈伤组织百分率,K+、Na+含量;在300 mmol/L NaCl胁迫下,测定了rn变异体、rh变异体和对照愈伤组织的生长量及脯氨酸含量。结果表明,rh变异体愈伤组织的分化频率高于rn变异体,Hyp的选择频率是NaCl的2倍左右;在不同浓度NaCl胁迫下,rh变异体具有较高的愈伤组织存活率和K+、Na+含量;在300 mmol/L NaCl胁迫下,rh变异体的生长量和脯氨酸含量均最高,表现出了较好的耐盐性。 |
| 关键词: 玉米 耐盐愈伤组织变异体 植物耐盐性 |
| DOI： |
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| 基金项目:河南省科技攻关项目（991160103） |
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| Selection of salt-tolerant callus variants in maize and analysis on salt-tolerance |
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| Abstract: |
| In order to obtain salt-tolerant callus variants in maize,the embryogenic calli were induced from immature embryos of 4 maize genotypes that were suitable for tissue culture.After 5 generations culture,salt-tolerant calli variants were obtained through three continuous selection with 200 mmol/L NaCl and 5.5 mmol/L Hyp,called rn variant and rh variant respectively,at the same time,callus from basal subculture medium was used as CK.Through one generation back-culture,under 200,250 and 300 mmol/L NaCl stress,callus survival ratio,embryogenic callus proportion and K+ and,Na+ contents of rn variant,rh variant and CK were counted or tested;growth quantity and free proline content of rn variant,rh variant and CK were tested under 300 mmol/L NaCl stress.The results showed that the differentiation frequency of rh variant was higher than that of rn variant;the selection frequency of Hyp was twice as much as that of NaCl.The rh variant had higher callus survival ratio and the contents of K+ and Na+ under different concentration salt stress;growth quantity and free proline content of rh variant under 300 mmol/L NaCl stress were the largest,showing stronger salt-tolerance. |
| Key words: maize,salt-tolerant callus variants,plant salt-tolerance |
