| 摘要: |
| 【目的】探讨17β-雌二醇（E2）上调绵羊输卵管上皮细胞SBD-2表达的可能信号通路。【方法】分离培养绵羊输卵管上皮细胞，将第2代输卵管上皮细胞分为E2(10-8 mol/L)组、雌激素受体拮抗剂ICI182780(10-7 mol/L)组、PKA 阻断剂KT-5720(1 μmol/L)组、PKC 阻断剂H-7(50 μmol/L)组、NF-κB阻断剂PDTC(50 μmol/L)组及空白对照(Control)组，在不同阻断剂组加入各自阻断剂干预绵羊输卵管上皮细胞1 h后，在各阻断剂组和E2组中再加入10-8 mol/L 17β-雌二醇培养6 h,然后采用实时荧光定量RT-PCR方法检测各组SBD-2 mRNA表达水平的变化。【结果】10-8 mol/L 17β-雌二醇可显著上调绵羊输卵管上皮细胞SBD-2 mRNA的表达(P＜0.05)；雌激素受体拮抗剂ICI182780、NF-κΒ阻断剂PDTC、PKC阻断剂H-7均可阻断17β-雌二醇对SBD-2的上调作用，PKA 阻断剂KT-5720对17β-雌二醇介导的SBD-2上调无明显影响。【结论】17β-雌二醇上调绵羊输卵管上皮细胞SBD-2的表达由雌激素核受体、NF-κΒ、PKC信号转导通路所介导，而PKA不参与17β-雌二醇对SBD-2的上调作用。 |
| 关键词: 17β-雌二醇 输卵管上皮细胞 SBD-2 信号通路 |
| DOI： |
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| 基金项目:国家自然科学基金项目(31060328) |
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| Up-regulation mechanism of 17β-estradiol on β-Defensin-2 (SBD-2) in ovine oviduct epithelial cells |
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| Abstract: |
| 【Objective】This study aimed to investigate the mechanisms involving in the inhibitory effect of 17β-estradiol (E2) on β-Defensin-2 (SBD-2) in ovine oviduct epithelial cells.【Method】Ovine oviduct epithelial cells were isolated and cultured,and then the second generation cells were divided into 17β-estradiol (10-8 mol/L) group,estrogen nuclear receptor antagonists ICI182780 (10-7 mol/L) group,PKA antagonist KT-5720 (1 μmol/L) group,PKC antagonist H-7 (50 μmol/L) group,nuclear factor kappa B antagonist PDTC (50 μmol/L) group and control group (Control).Different antagonists were added to each antagonist group to interfere ovine oviduct epithelial cells for 1 h,and then 17β-estradiol (10-8 mol/L) was added to each antagonist group and E2 group for 6 h.Real time PCR was used to detect the changes of SBD-2.【Result】The expression of SBD-2 mRNA was significantly up-regulated by 17β-estradiol (10-8 mol/L) (P<0.05).The effect was abrogated by ICI182780,PKC antagonist H-7 and NF-κΒ antagonist PDTC while PKA antagonist KT-5720 had no influence.【Conclusion】17β-estradiol induces SBD-2 mRNA expression in ovine oviduct epithelial cells,and the induction is mediated by signaling proteins of estrogen nuclear receptor,PKC and NF0κΒ rather than PKA. |
| Key words: 17β-estrodiol oviduct epithelial cells SBD-2 signaling pathways |
