| 摘要: |
| [目的]研究中国荷斯坦奶牛Leptin基因第2外显子的多态性,分析其多态性与产奶性状的关系.[方法]以中国荷斯坦奶牛为材料,利用PCR-SSCP技术对瘦素基因(Leptin)第2外显子的遗传多态性及其与奶牛产奶性状的相关性进行了分析.[结果]中国荷斯坦奶牛Leptin基因第2外显子有2种等位基因A和B,等位基因频率分别为0.6975和0.3025,群体多态信息含量为0.3330,杂合度为0.4220,A等位基因是群体中的优势等位基因;BB型个体第1,2,3胎的乳脂率和乳蛋白率及第1,2胎的产奶量等产奶性状显著或极显著优于AA型(P＜0.01或P＜0.05);第1,2,3胎的产奶量及第1,2胎的乳脂率和第2胎的乳蛋白率也显著或极显著优于AB型(P＜0.01或P＜0.05).[结论]Leptin基因可用于中国荷期坦奶牛产奶性状的辅助选择. |
| 关键词: Leptin基因 中国荷斯坦牛 PCR-SSCP 产奶性状Leptin基因 中国荷斯坦牛 产奶性状 |
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| 基金项目:国家“十一五”奶业重大专项(2006BAD04A11)；农业科技跨越计划项目（2007-23）；西北农林科技大学拔尖人才支持计划项目 |
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| Study on the polymorphism in exon 2 of Leptin gene and its relationship with milk production traits in Chinese Holstein |
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| Abstract: |
| 【Obiective】 The polymorphism of exon2 of Leptin gene was studied and its association with milk production traits in Chinese Herstein Cattle was analyzed.【Method】 This study was designed to identify the polymorphism of exon 2 of Leptin gene and its relationship with milk production traits in 200 Chinese Holstein by PCR-SSCP.【Result】 The frequencies of alleles A and B were 0.697 5 and 0.302 5 respectively.Polymorphism information content was 0.333 0,and heterozygosity was 0.422 0.The allele A was the dominant allele in Chinese Holstein.Individuals with genotype BB had a significant higher average milk protein percentage and milk fat percentage for all three parities,and a significant higher average milk yield for the first parity and the second parity than that of genotype AA(P＜0.01 orP＜0.05).They also had a significant higher milk yield for all three parities,milk fat percentage for the first parity and the second parity,milk protein percentage for the second parity better than that of genotypeAB(P＜0.01 orP＜0.05).【Conclusion】 The results indicated that Leptin gene could be used as the molecular genetic marker to select the milk production traits in cattle. |
| Key words: Leptin gene Chinese Holstein PCR-SSCP Milk production trait |
