| 摘要: |
| 【目的】 研究双酚A（BPA）对小鼠肾组织的毒性效应。【方法】 将BPA溶于金龙食用油中，备用。将20只小鼠随机分为5组：Ⅰ组为对照组，注射食用油1 mL/只；Ⅱ～Ⅴ组为药物处理组，分别注射0.1，0.5，1或2 mg/mL的BPA 1 mL/只，每5 d注射1次，连续注射3次。于末次注射后第5, 10, 15和20 天各组分别取1只小鼠处死，取肾脏，用多聚甲醛固定，常规方法制备切片，免疫组织化学染色显微观察β-actin的分布定位，分析BPA对小鼠肾组织β-actin阳性细胞表达的影响。【结果】 第5～15天，0.1和0.5 mg/mL BPA处理组小鼠肾组织β-actin阳性细胞数量与对照组差异不显著,1和2 mg/mL BPA处理组小鼠肾组织阳性细胞数量与其他3组差异显著；第20天，各BPA处理组小鼠β-actin阳性细胞数量与对照组差异显著。【结论】 小鼠肾组织中β-actin阳性细胞随BPA剂量的增加与用药时间的延长而减少。 |
| 关键词: 小鼠 双酚A 环境雌激素 免疫组织化学 β-actin |
| DOI： |
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| 基金项目:陕西省科技厅自然科学基金项目（SJ08C106）；陕西省教育厅自然科学基金项目（08JK501）；延安市2007年自然科学专项基金项目 |
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| Toxicity of systematic bisphenol A to mice kidney tissue |
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| Abstract: |
| 【Objective】 The toxicity of intraperitoneal injection of bisphenol A (BPA) of different doses to kidney tissue of mice was evaluated with the change of β-actin expression.【Method】 BPA was resolved in Jinlong plant oil. 20 mice were divided into 5 groups.One group injected with the plant oil was used as control.The other groups were injected with different doses of BPA (0.1,0.5,1 or 2 mg/mL per mice) at intervals of 5 d for 3 times respectively.After the injection of BPA or oil for 5,10,15 and 20 d,the mice were killed respectively.Then,each mouse kidney tissue was removed and fixed in polyformaldehyde.The change of β-actin expression kidney tissue was observed by immunohistochemical staining.【Result】 Compared with control group, after treatment with BPA from 5 d to 15 d,β-actin expression showed no change with the lower dose of BPA at 0.1 and 0.5 μg/mL.However,it was decreased significantly with the higher dose of BPA at 1 and 2 mg/mL.After the treatment with BPA for 20 d,the β-actin expression in all of groups treated with BPA was reduced greatly.【Conclusion】 These results indicated that the number of β-actin-stained positive kidney cells was reduced by bisphenol A at a dose-dependent and time-dependent manner,and thus the systematic bisphenol A showed the strong toxicity of mice kidney tissue. |
| Key words: mice BPA environmental estrogen immunohistochemistry β-actin |
