The present study was conducted to compare the muscle composition of wild discus fish Symphysodon sp., and cultured discus fish fed beef heart burger diet and formula diet. Results showed that crude protein content in muscle of wild discus fish was significantly higher, and crude fat content was significantly lower than that in cultured discus fish (P<0.05). There was no significant difference in moisture and ash contents among the three groups (P>0.05). Seventeen kinds of amino acids were measured in the muscle of the three types of fish. Glu had the highest value among all amino acids, and Lys had the highest value among essential amino acids. Both wild and cultured discus fishes had 19 kinds of fatty acids, which shared the similar fatty acids mode. C16: 0, C18: 1 and C20: 4 had the highest level in SFA, MUFA, and PUFA, respectively. The EPA and DHA contents in three discus fishes ranged from 0.93% to 1.86% and from 1.57% to 2.35%, respectively. The result provided the academic basis to infer the nutritional requirement and develop formulated feed for discus fish.