Growth traits and genetic parameters of hybrid families in Pinctada martensii
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摘要: 【目的】探究不同杂交策略下马氏珠母贝二元杂交子代的生长性状和养殖存活情况,为广西马氏珠母贝群体杂交育种配套系构建及选择育种提供参考依据。【方法】以马氏珠母贝海选1号(H)自育F4代群体和北海野生群体(Y)自繁F1代群体为亲本,通过二元杂交构建36个正反杂交家系,并以H亲本和Y亲本的自繁群体F1代分别构建对照群组(PH和PY);各群组经海区养殖14个月后采集壳长(SL)、壳高(SH)、壳宽(SW)和体质量(BW)等数据,利用混合线性模型(动物模型)进行生长性状遗传参数估计。【结果】在H(♂)×Y(♀)杂交子代中,SL、SH、SW和BW的遗传力(h2)分别为0.241、0.149、0.220和0.062,遗传相关系数(γg)范围为0.894~0.964,表型相关系数(γp)范围为0.558~0.865;在Y(♂)×H(♀)杂交子代中,SL、SH、SW和BW的h2分别为0.110、0.156、0.121和0.067,γg范围为0.981~0.989,γp范围0.603~0.881;2个杂交子代的生长性状表现为中低遗传力,且遗传相关高于表型相关。Y(♀)和Y(♂)群体的一般配合力(GCA)较高,说明更适合用于杂交配套;H(♂)×Y(♀)杂交子代中SL、SH和SW的特殊配合力(SCA)相对较高,且均为中低遗传力。在2个杂交子代中,4个生长性状的杂种优势分别为-0.02%~2.82%和-0.03%~0.27%,均以SL的杂种优势最高、SW的杂种优势最低。在H(♂)×Y(♀)杂交子代中SL、SH和BW等3个生长性状的杂种优势家系率为50.00%~72.22%,优势家系选择率≥50.00%;养殖存活性状的超亲优势家系率为19.44%,杂种优势家系率为61.11%,2个杂交子代和PY群体均表现出优势存活性状。【结论】H(♂)×Y(♀)杂交子代具有生长性状优势,Y(♂)×H(♀)杂交子代具有存活性状优势,2个杂交子代均存在综合杂种优势,可进行一般杂交育种应用;北海野生群体亲本的GCA较高,其SL和SH的遗传力及与BW的遗传相关也相对较高,在进行广西马氏珠母贝群体杂交制种时可考虑以野生群体子代作为专门化亲本来源,且对2龄亲本性状选择上宜优先考虑SL和SH。Abstract: 【Objective】To explore the growth and survival of two-way crossing hybrid of Pinctada martensii under different crossbreeding strategies,and to offer reference for the construction of cross breeding mating lines and selective breeding of P. martensii in Guangxi.【Method】36 hybrid families were esta blished in a complete diallel hybridization design which parents came from a F4 generation Haixuan No.1 stock(H)and a F1 local wild stock(Y)from Beihai. Control groups(PH and PY)were constructed from the F1 generation of the H and Y parents. After 14 month’s mariculture, growth characters were compared among hybrid families(HY,YH)and purebred populations(PH,PY),genetic parameters of growth traits such as shell length(SL),shell height (SH),shell width(SW),and body weight(BW)were estimated by a mixed linear model(animal model).【Result】Heritabilities(h2)of SL,SH,SW and BW of HY group were 0.241,0.149,0.220 and 0.062,respectively,while the range of genetic correlation(γg)and phenotypic correlation(γp) coefficient were 0.894-0.964 and 0.558-0.865. Heritabilities of SL,SH,SW and BW of YH group were 0.110,0.156, 0.121 and 0.067,respectively,while the range of genetic correlation and phenotypic correlation coefficient were 0.981- 0.989 and 0.603-0.881. The outcomes indicated that both two hybrid groups had medium-low heritabilities,and genetic correlation coefficients were higher than phenotypic correlation coefficients. The general combining ability(GCA)of Y (♀)and Y(♂)populations was high,indicating that Y(♀)and Y(♂)were more suitable for cross matching. The special combining ability(SCA)of SL,SH and SW of H(♂)×Y(♀)hybrids were high and had low-medium heritabilities. The growth heterosis(%)of four growth traits in the twohybrids ranged from -0.02% to 2.82% and -0.03% to 0.27%,respectively. SL had the highest heterosis,while SW had the lowest heterosis among all the groups. The proportion of families with heterosis in SL,SH and BW of H(♂)×Y(♀)hybrids ranged from 50.00% to 72.22%,and the selection rate of superior families was ≥50.00%. The proportion of over parent heterosis families in survival traits was 19.44%,while the proportion of heterosis families was 61.11%. Both the two hybrid groups and PY population showed advantageous survival rates. 【Conclusion】H(♂)×Y(♀)hybrids have advantage in growth,while Y(♂)×H(♀)hybrids have advantage in survival rate, and the two hybrid combinations all showcomprehensive heterosis,which can be used in general cross breeding. The offspring of Beihai wild population which with relative high GCA,heritabilities of SL and SH and high genetic correlation with BW,can be considered as specialized parental sources in the cross breeding of Guangxi P. martensii population. SL and SH should be given priority in the trait selection of 2-year-old parent individuals.
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Key words:
- Pinctada martensii /
- two-way cross /
- genetic parameters /
- combining ability /
- heterosis
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陈健,白丽蓉,罗会,喻达辉. 2020. 2种贝龄合浦珠母贝数量性状的相关与通径分析[J]. 南方农业学报,51(10):2557-2564.[Chen J,Bai L R,Luo H,Yu D H. 2020. Correlation and path analysis of quantitative traits of pearl oyster Pinctada fucata at two different ages[J]. Journal of Southern Agriculture,51(10):2557-2564.]doi: 10.3969/j.issn.2095-1191.2020.10.029. 陈明强,刘宝锁,严俊贤,郭华阳,吴开畅,李有宁. 2016. 合浦珠母贝4种壳色选育系主要性状的比较分析[J]. 南方水产科学,12(5):118-122.[Chen M Q,Liu B S,Yan J X, Guo H Y,Wu K C,Li Y N. 2016. Comparison analysis of major traits among four shell color selective lines of pearl oyster(Pinctada fucata)[J]. South China Fisheries Science,12(5):118-122.]doi:10.3969/j.issn.2095-0780. 2016.05.015. 陈小宏,蔡强,陈海进,赵军,徐会平. 2018. 马氏珠母贝"海选 1号"和对照群体生长性状的比较[J]. 安徽农业科学,46(1):84-86.[Chen X H,Cai Q,Chen H J,Zhao J,Xu H P. 2018. Comparison of growth traits between"Haixuan No.1"breed and control groups of pearl oyster Pinctada fucata martensii[J]. Journal of Anhui Agricultural Sciences,46(1):84-86.]doi:10.3969/j.issn.0517-6611.2018.01. 025. 黄桂菊,陈飞飞,刘宝锁,范嗣刚,刑孔武,喻达辉. 2016. 合浦珠母贝3个养殖群体双列杂交F1代的生长和遗传分析[J]. 海洋科学,40(2):26-34.[Huang G J,Chen F F,Liu B S,Fan S G,Xing K W,Yu D H. 2016. Growth performances and genetic analysis of complete diallel crosses of Pinctada fucata based on three cultured populations[J]. Marine Sciences,40(2):26-34.]doi:10.11759/hykx2014 0819003. 李奉令. 2019. 关于用育种值提升一般配合力分析水平的研究[J]. 种子科技,37(1):92-93.[Li F L. 2019. Study on improving the analysis level of general combining ability by breeding value[J]. Seed Science & Technology,37(1):92-93.]doi: 10.3969/j.issn.1005-2690.2019.01.077. 李俊辉,刘青云,杜晓东,邓岳文,王庆恒. 2016. 马氏珠母贝养殖群体早期生长阶段的遗传多样性与有效亲本数量估计[J]. 广东海洋大学学报,36(4):12-16.[Li J H,Liu Q Y,Du X D,Deng Y W,Wang Q H. 2016. Genetic diversity and effective population size estimate in the stock of pearl oyster Pinctada fucata martensii at early growth stages[J]. Journal of Guangdong Ocean University,36(4):12-16.]doi: 10.3969/j.issn.1673-9159.2016.04.003. 李镒民,丁琳琳,谭杰,苏晓盈,刘志刚,刘付少梅. 2019. 马氏珠母贝F8代黑壳色选育系群体与普通养殖群体遗传多样性分析[J]. 基因组学与应用生物学,38(11):4961-4967.[Li Y M,Ding L L,Tan J,Su X Y,Liu Z G,Liufu S M. 2019. Genetic diversity analysis of the eighth generation black-shell colored line population and the ordinary cultured population of Pinctada martensii[J]. Genomics and Applied Biology,38(11):4961-4967.]doi:10.13417/j.gab. 038.004961. 林元震,陈晓阳. 2014. R与ASReml-R统计分析教程[M]. 北京:中国林业出版社.[Lin Y Z,Chen X Y. 2014. R & ASReml-R statistical analysis tutorial[M]. Beijing:China Forestry Publishing House.] 楼允东. 2001. 鱼类育种学[M]. 北京:中国农业出版社.[Lou Y D. 2001. Fish breeding[M]. Beijing:China Agriculture Press.] 罗会,刘宝锁,黄桂菊,范嗣刚,喻达辉. 2016. 合浦珠母贝完全双列杂交组合生长性状比较[J]. 海洋渔业,38(6):645-652.[Luo H,Liu B S,Huang G J,Fan S G,Yu D H. 2016. Comparison of growth traits in Pinctada fucata for complete diallel crosses groups[J]. Marine Fisheries, 38(6):645-652.]doi: 10.13233/j.cnki.mar.fish.2016.06.010. 孟乾,李琪,王许波,张景晓,于瑞海. 2018. 长牡蛎'海大1号'生长及育种性能分析[J]. 中国水产科学,25(2):263-268.[Meng Q,Li Q,Wang X B,Zhang J X,Yu R H. 2018. Analysis of growth performance and breeding value of the pacific oyster'Haida No.1'[J]. Journal of Fishery Sciences of China,25(2):263-268.]doi:10.3724/SP.J. 1118.2018.16371. 孙静,陈明强,魏海军,邓正华,李有宁,金朝焕,王继金,王雨,石承开,黄超旬. 2020. 不同地理群体合浦珠母贝双列杂交子代数量性状比较及其相关和通径分析[J]. 南方农业学报,51(6):1451-1461.[Sun J,Chen M Q,Wei H J,Deng Z H,Li Y N,Jin C H,Wang J J,Wang Y,Shi C K,Huang C X. 2020.Quantitative traits of diallel hybrid progeny of Pinctada fucata from different geogra -phic populations and correlation and path analysis[J]. Journal of Southern Agriculture,51(6):1451-1461.]doi: 10.3969/j.issn.2095-1191.2020.06.027. 谭杰,孙宗红,陶雅晋,姚高友,刘付少梅,刘锦上,刘志刚. 2018. 马氏珠母贝F8代4种壳色选育群体的EST-SSR遗传多样性分析[J]. 水产科学,37(4):505-511.[Tan J,Sun Z H,Tao Y J,Yao G Y,Liufu S M,Liu J S,Liu Z G. 2018. Genetic diversity analysis of selective breeding F8of pearl oyster Pinctada martensii with four shell colors by EST-SSR markers[J]. Fisheries Science,37(4):505-511.]doi: 10.16378/j.cnki.1003-1111.2018.04.012. 陶后全,吴灶和,白成,刘冠,刘志刚. 2014. 马氏珠母贝早期生长性状的遗传参数估计[J]. 广东海洋大学学报,34(3):10-16.[Tao H Q,Wu Z H,Bai C,Liu G,Liu Z G. 2014. Heritability estimates for growth straits in the earlier larva stage of Pinctada martensii[J]. Journal of Guangdong Ocean University,34(3):10-16.]doi:10.3969/j.issn. 1673-9159.2014.03.003. 王祎哲,邓正华,王雨,李有宁,于刚,黄桂菊,陈明强. 2018.合浦珠母贝不同壳色选育系F6数量性状的相关性和通径分析[J]. 中国水产科学,25(5):988-997.[Wang Y Z, Deng Z H,Wang Y,Li Y N,Yu G,Huang G J,Chen M Q. 2018. Correlation and path analyses of quantitative traits in different shell color F6 lines of pearl oyster(Pinctada fucata)[J]. Journal of Fishery Sciences of China,25(5):988-997.]doi: 10.3724/SP.J.1118.2018.17446. 杨创业,吴丹阳,王庆恒,邓岳文,杜晓东. 2015. 马氏珠母贝生长性状遗传力估计[J]. 中国农学通报,31(23):25-29.[Yang C Y,Wu D Y,Wang Q H,Deng Y W,Du X D. 2015. Heritability estimation for growth traits of Pinctada martensii[J]. Chinese Agricultural Science Bulletin, 31(23):25-29.] 尹立鹏,邓岳文,杜晓东,王庆恒. 2012. 贝龄对马氏珠母贝植核贝生长、成活率和育珠性状的影响[J]. 中国水产科学,19(4):715-720.[Yin L P,Deng Y W,Du X D,Wang Q H. 2012. Effects of age on growth,survival and pearl production and traits in pearl oyster Pinctada martensii[J]. Journal of Fishery Sciences of China,19(4):715-720.]doi: 10.3724/SP.J.1118.2012.00715. 张善发,贺承章,李俊辉,邓岳文,杨创业. 2020. 马氏珠母贝金黄壳色选育群体与养殖群体不同组织中矿物质元素的分析与评价[J]. 海洋通报,39(3):357-362.[Zhang S F,He C Z,Li J H,Deng Y W,Yang C Y. 2020. Analysis and evaluation of mineral contents in different tissues of pearl oyster Pinctada fucata martensii between the yellow colored line and cultured stock[J]. Marine Science Bulletin,39(3):357-362.]doi:10.11840/j.issn.1001-6392. 2020.03.010. 赵云翔,刘沁沅,高宁,郑伟,李私丞,张从林,刘小红. 2019.仔猪均匀度及相关繁殖性状的遗传参数估计与分析[J]. 南方农业学报,50(7):1620-1624.[Zhao Y X,Liu Q Y, Gao N,Zheng W,Li S C,Zhang C L,Liu X H. 2019.Genetic parameters estimation and analysis of litter uniformity and other reproductive traits in sow[J]. Journal of Southern Agriculture,50(7):1620-1624.]doi: 10.3969/j.issn.2095-1191.2019.07.29. 郑静静,刘建勇,刘加慧,张嘉晨,袁瑞鹏. 2016. 凡纳滨对虾(Litopenaeus vannamei)不同生长阶段体重的遗传参数和育种值估计[J]. 海洋与湖沼,47(5):1005-1012.[Zheng J J,Liu J Y,Liu J H,Zhang J C,Yuan R P. 2016. Genetic parameterization and breeding value on body weight of Litopenaeus vannamei in different grouth stages[J]. Oceanologia et Limnologia Sinica,47(5):1005-1012.]doi: 10.11693/hyhz20160400075. 邹杰,彭慧婧,张守都,张悦,杨家林. 2020. 施氏獭蛤壳体表型性状对体质量的影响分析[J]. 水产科学,39(4):573-578.[Zou J,Peng H J,Zhang S D,Zhang Y,Yang J L. 2020. Effects of shell phenotypic traits on body weight of clam Lutraria sieboldii[J]. Fisheries Science,39(4):573-578.]doi: 10.16378/j.cnki.1003-1111.2020.04.015. 邹杰,张守都,彭慧婧,郑德斌. 2021. 施氏獭蛤早期生长性状遗传参数和育种值估计[J]. 海洋科学,45(2):99-105.[Zou J,Zhang S D,Peng H J,Zheng D B. 2021. Estimation of genetic parameters and breeding values of early growth traits of Lutraria sieboldii[J]. Marine Sciences, 45(2):99-105.]doi: 10.11759/hykx20200506001. Abdalla M M F,Shafik M M,Attia M S,Ghannam H. 2017.Heterosis,GCA and SCA effects of diallel-cross among six Faba Bean(Vicia faba L.)genotypess[J]. Asian Research Journal of Agriculture,4(4):1-10. doi: 10.9734/ARJA/2017/32291. Muhammad G,Fujimura T,Sahidin A,Komaru A. 2021. The influence of donor and recipient oyster red and blue shell nacre interference color on Pinctada fucata martensii pearl quality[J]. Aquaculture,543:736947. doi: 10.1016/j.aquaculture.2021.736947. Murtadha M A,Ariyo O J,Alghamdi S S. 2016. Analysis of combining ability over environments in diallel crosses of maize(Zea mays)[J]. Journal of the Saudi Society of Agricultural Sciences,17(1):69-78. doi:10.1016/j. jssas. 2016.01.004.
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