| 不同抗性黄瓜自交系接种白粉病原菌后生理特性的变化 |
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| 引用本文：蒋道伟，司龙亭.不同抗性黄瓜自交系接种白粉病原菌后生理特性的变化[J].西北农业学报,2010,19(8):161~165 |
| DOI:10.7606/j.issn.1004-1389.2010.08.032 |
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| 基金项目:辽宁省“十五”科技攻关项目（2002215004）。 |
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| 中文摘要:对不同抗性黄瓜自交系接种白粉病原菌后，测定叶片中与抗性有关的叶绿素、可溶性糖、可溶性蛋白和4种防御酶活性。结果表明，叶绿素含量在接种后均呈先增加后减少的趋势，抗病自交系增加幅度较大，叶绿素含量高于感病自交系，黄瓜抗白粉病能力与叶绿素含量存在一定程度的正相关；可溶性糖含量在接种后先降低 后升高 再降低 再升高，抗病自交系可溶性糖含量高于感病自交系；可溶性蛋白含量在接菌后均逐渐升高，感病自交系的峰值出现早于抗病自交系，感病自交系对病原菌较敏感，抗病自交系对病菌的侵染反应呈一定程度的负相关；4种酶的活性在接菌后都有不同程度的提高，抗病自交系酶活性增加幅度高于感病自交系，说明这4种酶在黄瓜对白粉病的抗性机制中起了重要作用。 |
| 中文关键词:黄瓜自交系 白粉病原菌 生理变化 |
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| Changes of Physiological Characteristics in Different Cucumber Breeding Lines Infected by Sphaerotheca fuliginea |
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| Abstract:The content of chlorophyll, soluble sugar, and soluble protein, and the activity of four protective enzymes in the leaves of cucumber breeding lines inoculated with Sphaerotheca fuliginea were measured. The results showed that chlorophyll content increased firstly then decreased both in the resistant and susceptible lines after inoculation. However, the level and increment of chlorophyll content were higher in the resistant lines than in the susceptible lines, which implied that the cucumber resistance to powdery mildew was positive correlation with chlorophyll content. Soluble sugar content had a basically same trend of reduce increase reduce increase after inoculation. Soluble sugar content of the resistant lines was higher than that of the susceptible lines. Soluble protein content increased both in the resistant and susceptible lines. The peak of soluble protein content appeared earlier in the susceptible lines than in the resistant lines, which indicated that the susceptible lines were more sensitive to the pathogen than the resistant lines. Negative relationship was found between disease resistance and Sphaerotheca fuliginea infection response. The activities of four enzymes, including polyphenoloxidase (PPO), peroxidase (POD), phenylalanine ammonia lyase (PAL) and catalase (CAT), increased with different amplitude after inoculation, the increment amplitude of the resistant lines was larger than that of the susceptible lines, this illustrated that the four enzymes play important roles in resistance mechanism to Sphaerotheca fuliginea. |
| keywords:Cucumber breeding lines Sphaerotheca fuliginea Physiological changes |
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