| 导入南瓜DNA选育抗枯萎病西瓜新种质的研究 |
| 投稿时间：2001-10-25 点此下载全文 |
| 引用本文：王浩波,林茂,杨坤,戴祖云,程国旺,余增亮.导入南瓜DNA选育抗枯萎病西瓜新种质的研究[J].西北农业学报,2002,11(1):24~27 |
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| 摘要点击次数: 38 |
| 全文下载次数: 20 |
| 作者 | 单位 | | 王浩波 | 中国科学院等离子体所丰乐种业离子束生物工程中心, 安徽合肥, 230031 | | 林茂 | 中国科学院等离子体所丰乐种业离子束生物工程中心, 安徽合肥, 230031 | | 杨坤 | 中国科学院等离子体所丰乐种业离子束生物工程中心, 安徽合肥, 230031 | | 戴祖云 | 中国科学院等离子体所丰乐种业离子束生物工程中心, 安徽合肥, 230031 | | 程国旺 | 中国科学院等离子体所丰乐种业离子束生物工程中心, 安徽合肥, 230031 | | 余增亮 | 中国科学院等离子体所丰乐种业离子束生物工程中心, 安徽合肥, 230031 |
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| 基金项目:国家自然科学基金项目资助(编号:119890300) |
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| 中文摘要:采用子房注射法将南瓜总DNA导入西瓜,企望使西瓜和南瓜实现分子水平上的远缘杂交,从而获得整合有控制抗病的南瓜DNA片段而不改变受体西瓜农艺性状的西瓜新种质.用该方法在西瓜自交授粉后24h从子房顶端将15μl南瓜DNA注入胚囊中,经病圃田间筛选和6代自交纯化已获得5份稳定西瓜新材料.经苗期人工接种和病圃田间自然抗病鉴定,其抗病性明显提高.所获抗病材料的品质与受体无明显差异.其中2份材料的果皮色泽和花纹是不同于供受体的新变异类型.试验表明,子房注射法将外源DNA导入西瓜是一种简便实用的分子育种方法,是解决西瓜抗病种质资源匮乏和遗传基础狭窄的有效途径. |
| 中文关键词:西瓜 外源DNA 抗病育种 枯萎病 |
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| Study on the Selection of New Watermelon Germplasm for Resistance to Fusarium Wilt Disease by Introducing Pumpkin DNA |
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| Abstract:The DNA extracted from the pumpkin leaves was in troduced in to the embryos of watermelon line through embryo-injection after self-pollination.The aim of this study was that watermelon and pumpkin should be hybridized in molecular level, and the new watermelon germplasm should be obtained having the pumpkin DNA fragments with resistance to fusarium wilt and not altering the agronomic character of receptor watermelon.In this article the 15μl extracted liquid of pumpkin DNA was in jected in to receptor watermelon's embryos after self-pollination 24 h.Five new watermelon germ plasm of resistance to Fusarium oxysporum sp.niveum had been selected by six generation selfing in the wiltd isease field.Two of the five watermelon germ plasm have new special rind color and strip, which is different from the receptor watermelon's and the pumpkin's.The fruits quality of five watermelon varieties obtained by exogenous DNA in troducing have no differentiation from the receptor watermelon line's.This study shows that the embryo in jection of in troducing exogenous DNA was a convenient and practical method, especially on solving the problem of watermelon genetic germplasm being narrow and limited. |
| keywords:Watermelon Exogenous DNA Introduction Breeding for diseases resistance Fusarium wilt |
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