| 牦牛和犏牛 DDX25/ GRTH基因序列及其在睾丸组织的表达水平 |
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| 引用本文：张思源，柴志欣，彭娅林，钟金城.牦牛和犏牛 DDX25/ GRTH基因序列及其在睾丸组织的表达水平[J].西北农业学报,2015,24(10):1~9 |
| DOI:10.7606/j.issn.1004-1389.2015.10.001 |
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| 基金项目:西南民族大学研究生创新科研项目（CX2014SZ127）；西南民族大学研究生学位点建设项目（2014XWD-S071007）。 |
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| 中文摘要:为了解 DDX25基因的结构、功能及对犏牛雄性不育的影响。采用RT-PCR技术克隆获得牦牛和犏牛的 DDX25基因，对核苷酸序列和氨基酸序列进行生物信息学分析，运用实时荧光定量PCR技术检测睾丸组织 DDX25 mRNA的表达情况。结果表明，牦牛和犏牛 DDX25基因编码区序列全长均为1 452 bp，编码483个氨基酸，氨基酸存在19个磷酸化位点，无信号肽。牦牛睾丸组织 DDX25基因表达水平显著高于犏牛， DDX25基因在犏牛睾丸组织的低表达与其雄性不育存在一定关系，该基因可作为研究犏牛雄性不育的候选基因。 |
| 中文关键词:牦牛 犏牛 雄性不育 DDX25/GRTH 表达水平 |
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| Sequencing and Expression of DDX25/GRTH mRNA in Yak and Cattle-yak Testis |
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| Abstract:To understand the structure of DDX25, gene function and its impact on cattle-yak male sterility, DDX25 gene was cloned by RT-PCR from cattle-yak and yak, conducting bioinformatics analysis based on the sequences of nucleotide and amino acid, and DDX25 mRNA expression in testis was detected by Real-time PCR.The results showed that DDX25 gene coding sequences in yak and cattle-yak is the full length of 1 452 bp, encoding 483 amino acids with 19 phosphorylation sites, without signal peptide. DDX25 gene expression level in testis of yak were significantly higher than in testis of cattle-yak.Low expression of DDX25 gene in testis of cattle-yak has a certain relationship with their male sterility, so the gene can be used as a candidate gene for male sterility cattle-yak. |
| keywords:Yak Cattle-yak Male sterility DDX25/GRTH Expression level |
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