| 博落回中生物碱的超声提取和分离 |
| 投稿时间：2005-05-27 修订日期：2005-07-01 点此下载全文 |
| 引用本文：王欣,周乐,季春燕,刘拉平.博落回中生物碱的超声提取和分离[J].西北农业学报,2005,14(5):118~120,124 |
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| 作者 | 单位 | E-mail | | 王欣 | 西北农林科技大学生命科学学院, 陕西, 杨凌, 712100 | | | 周乐 | 西北农林科技大学生命科学学院, 陕西, 杨凌, 712100 | betterzl@163.com | | 季春燕 | 西北农林科技大学生命科学学院, 陕西, 杨凌, 712100 | | | 刘拉平 | 西北农林科技大学测试中心, 陕西, 杨凌, 712100 | |
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| 中文摘要:运用超声波辅助提取技术,通过L9(3)4正交试验考察了乙醇浓度(A)、液固比(B)和超声时间(C)3个因素对博落回生物碱提取的影响,同时还研究了D101大孔树脂对粗提液中生物碱的吸附和分离效果。结果表明最佳提取工艺为A3B1C3,即用9倍量的90%的乙醇超声提取45min。D101大孔树脂能从博落回的pH2～3的盐酸提取液中以游离碱形式全部吸附其生物碱,分别采用50%乙醇和丙酮洗脱能有效地将极性和弱极性生物碱分离开来。 |
| 中文关键词:博落回 超声提取 生物碱 D101大孔树脂 正交试验 |
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| Ultrasonic Extraction and Separation of Alkaloids from Macleaya cordata |
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| Abstract:The effects of alcohol concentration,liquid-solid ratio and ultrasonic times on the extraction yields of alkaloids from Macleaya cordatu were studied by means of L9(3)4,orthogonal test,and the effects of adsorption and separation of alkaloids in extract with macroporous restns D101 were investigated in the present paper.It was found that the optimal extraction conditions were A3N1C3,i.e.9 times amount of 90% ethanol for ultrasonic extracting 45 minutes that all alkaloids in the hydrochloric acid solution of pH2~3 were abstracted in the form of free base,and that the polar alkaloids and weak polar alkaloids were separated efficienty by means of 50% ethanol elution and acetone respectively. |
| keywords:Mucleuyu cordutu (willd.)R. Br Ultrasonic extraction Alkaloid Macroporous resins D101 Orthogonal test |
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