Planosol is one of the major low yield upland soils in the Northeast part of China.The albic horizon beneath the topsoil has low aeration,water permeability,and soil enzyme activity,this caused the topsoil suffer alternately excessive water and drought during the growing season,and its available layer for upland crops roots is only about 20 cm.The objective of this study was to investigate the effects of organic fertilizer and lime application on soil enzyme and yield of soybean in planosol.Four treatments,namely the conventional fertilizer application (CK),the conventional fertilizer application + lime(CK + Lime),the conventional fertilizer application+ organic fertilizer (CK + OF) and the conventional fertilizer application+lime+organic fertilizer (CK + Lime + OF) .Soil enzyme activities,including catalase,urease,intervase,were investigated at soybean maturity.The test fields were operated in the 853 farm,Heilongjiang province,and the indicator crop was soybean.The results showed that soil enzyme activity was CK + OF＞ CK + Lime+ OF ＞ CK ＞ CK + OF,and the soil organic was increased by organic fertilizer,the pH of soil was increased by lime.The three treatments increased soybean yield by 23.76 %，28.32%,13.07%,respectively.The organic fertilizer and lime increased yield of soybean by separate or combination application.