Rice is the main cereal crops in southern China，nutrient biofortification of Zn in rice grain has attracted great attention in recent years.Zn absorption and utilization of rice are affected by the interaction between Zn and phosphorus(P). The aim of study was to provide a theoretical basis on Zn absorption，accumulation and translocation of rice by rational fertilization.The study was based on a successive 22-years field experiment in the yellow paddy soil.Seven treatments were chosen from the experiment，including CK，N，NK，NPK，M，0.5MNP and MNPK.CK，N，NK had no P2O5 input，NPK，M and 0.5MNP had P2O5 input of 82.5，79.4 and 81.0 kg·hm-2，respectively，and MNPK had P2O5 input of 161.9 kg·hm-2.Soil and rice aboveground parts were sampled at tillering，flowering and maturity stages，and the contents of Zn in soil and in straw and grain of rice were measured.Zn accumulation and transfer characteristics were calculated.The soil available Zn(DTPA-Zn)content at different growth stages of rice was basically ranked as follows:MNPK≥M>0.5MNP>NPK≥CK>N，NK，indicating that long-term application of organic fertilizer improved soil Zn availability.Compared with the plant maturity stage in NPK treatment，Zn content in stem leaf and grain increased by -2.0%～44.0% and 15.7%～46.7% respectively in CK，N and NK treatment.Zn content in stem leaf and grain decreased by 22.0%～38.7% and 12.0%～19.8% respectively in MNPK treatment，and Zn content in grain increased by 6.4%～14.4% in M and 0.5MNP treatments.The P/Zn ratios in straw and grain in the maturity stage were 6.2～67.2 and 62.2～225.2，respectively，which increased with the increase of P application rate.There was no significant difference in Zn accumulation among different fertilization treatments.The Zn translocation amount and its contribution rate to grain were the highest in M treatment，and the lowest in N and NPK treatment，while Zn accumulation amount in after-anthesis period and its contribution rate to grain were the opposite.Correlation analysis and multivariate analysis of variance showed that，Zn content in stem leaf and grain was negatively correlated with P content and soil available P，DTPA-Zn content was mainly affected by the amount of manure，and Zn content in rice grain was mainly affected by the amount of P fertilizer.In conclusion，long-term P deficiency could increase the Zn content of rice grain，but seriously restricted the improvement of rice yield.Long-term high P application could increase the yield of rice grain，but reduced the Zn content in rice grain.Under suitable P application rate，long-term integrated fertilization with organic manure and chemical fertilizers could not only realize crop high yielding，but also improve the Zn content and activity in grain，which is an effective way to achieve high yielding and high quality of rice crop in yellow paddy soil.In addition，the effect of application of Zn fertilizer to the soil on increasing Zn content of rice grain is limited，so more attention should be paid to apply Zn fertilizer by foliar spray in order to further improve the Zn content in rice grain.