极大硬毛藻无性系对海水养殖废水中氮盐的去除效果
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国家重点研发计划(2017YFD0701700),广东省重点研发计划(2019B020215001)和国家海水鱼产业技术体系(CARS-47)


Removal efficiency of nitrogen salts by Chaetomorpha maxima in aquacultural wastewater
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    摘要:

    养殖水体中无机氮的高效去除是开展循环水养殖的重要保障条件之一。该论文研究了极大硬毛藻无性系(简称极大硬毛藻)对循环水养殖水体中无机氮盐的去除效率及特征。结果表明,当藻体密度为(10±1) g/L时,在一定浓度范围(氨氮:0~15 mg/L,亚硝酸盐氮:0~3 mg/L,硝酸盐氮:0~15 mg/L),极大硬毛藻对海水中3类无机氮盐的吸收速率随着时间变化即氮盐浓度降低而降低;其中藻类对氨氮的吸收速率变化较大,而对亚硝酸盐和硝酸盐的吸收速率相对稳定。当3种氮盐质量浓度为3 mg/L,极大硬毛藻首先选择吸收氨氮;当氨氮质量浓度降至1.5 mg/L时,藻开始吸收亚硝酸盐氮和硝酸盐氮。极大硬毛藻对人工模拟养殖废水中氨氮、亚硝酸盐氮和硝酸盐氮的15 h去除率可达到94.3%、100%、82.2%。该研究可为极大硬毛藻在循环水养殖水体净化的应用、养殖废水资源化利用和无害化处理技术的建立提供科学依据。

    Abstract:

    Abstract: The high efficiency in removing nitrogen salt is the key to insure the operation of recirculating aquacultural systems. The current study focused on the efficiency and the characteristic of Chaetomorpha maxima clones (hereinafter referred to as Chaetomorpha maxima) to remove the ammonia, the nitrite and the nitrate in seawater. The absorption efficiency of Chaetomorpha maxima to nutrients is mainly affected by the enzyme activity of algae and the concentration of nutrients in water. The absorption of NO3--N and NO2--N by Chaetomorpha maxima is more limited to its enzyme activity. The absorption of NH4+-N depends on the concentration of NH4+-N in water. Within a certain concentration (NH4+-N: 0-15 mg/L, NO2--N: 0-3 mg/L, NO3--N: 0-15 mg/L), the absorption rate of Chaetomorpha maxima on nitrogen salt suppressed with the reduction of nitrogen concentration in the water. The absorption rate of ammonia nitrogen by algae changed greatly. The final absorption rate of the 5, 10 mg/L group was close to 0, and that of the 15 mg/L group was 20×103 mg/(g·h). While the absorption rate of nitrite and nitrate is relatively stable. The final absorption rate of three groups of NO3--N is 20-30 mg/(g·h), and that of NO2--N was 2-4 mg/L. When the concentrations of three nitrogen salts were same (3 mg/L), Chaetomorpha maxima first absorbed NH4+-N, and the absorption rate on NH4+-N was notably higher than on NO2--N and NO3--N. There was no significant difference between NO2--N and NO3--N. However, when the NO2--N content was too low, the algae will preferentially absorb NO3--N. When ratio (g/L) of the fresh weight of Chaetomorpha maxima to water volume was 10∶1, the removal rates on NH4+-N, NO2--N and NO3--N of Chaetomorpha maxima in the artificial aquacultural wastewater (NH4+-N: 3.0±0.5 mg/L, NO2--N: 0.1±0.05 mg/L, NO3--N: 3.0±0.5 mg/L, PO43--P: 0.2±0.5 mg/L)were 94.3%, 100% and 82.2% in 15 hours. Although the removal rate of NH4+-N by Chaetomorpha maxima was high, it could not replace microorganism as the main unit of NH4+-N removal completely. In order to obtain sufficient ammonia NH4+-N as electron acceptor for microbial, the algae unit was suggested to be located behind the microbial unit. The practical application of algae treatment unit should consider comprehensively both the algae treatment efficiency and water renewal rate. In the actual production, the ratio between the fresh algae quality and the water volume can be adjusted according to the concentration of NO3--N in the water, so as to improve the removal rate of nitrate. The mass concentrations of NO3--N, NH4+-N, NO2--N in recirculating aquacultural systems were generally at 0-10, 0-5, 0-2 mg/L. Within the range of nitrogen salt concentration in circulating water system, three kinds of nitrogen salts will not cause adverse effects on algae. This study could provide data for the application of Chaetomorpha maxima clones in the treatment of aquacultural wastewater and promoted the utilization of aquaculture wastewater as a resource.

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李雪莹,李贤,王金霞,马晓娜,周浩然,徐建平,刘钦,李军.极大硬毛藻无性系对海水养殖废水中氮盐的去除效果[J].农业工程学报,2019,35(24):206-212. DOI:10.11975/j. issn.1002-6819.2019.24.025

Li Xueying, Li Xian, Wang Jinxia, Ma Xiaona, Zhou Haoran, Xu Jianping, Liu Qin, Li Jun. Removal efficiency of nitrogen salts by Chaetomorpha maxima in aquacultural wastewater[J]. Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE),2019,35(24):206-212. DOI:10.11975/j. issn.1002-6819.2019.24.025

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  • 收稿日期:2019-05-29
  • 最后修改日期:2019-11-28
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  • 在线发布日期: 2020-01-05
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