Abstract: Conservation tillage for paddy is very common in Southern China. However, problems such as bulk density and compaction increasing occurred for paddy fields with long-term conservation tillage, which was not favorable for paddy rice growth. In order to solve those problems, rotational tillage was introduced in this study. No-tillage(NT), rotary tillage(RT), and conventional tillage(CT) were conducted in the paddy field with seven years no-tillage in double rice cropping region, Hunan Province. And treatments were NT-NT, NT-RT and NT-CT, respectively. The tillage methods were the same of early rice and late rice. Results showed as follows: NT-RT and NT-CT treatments showed lower soil bulk density and higher water storage than NT-NT during the rice-growing stage. The soil saturated mass water content and bulk density showed inverse proportion under tillage treatments. The capillary porosity at 0～5 cm soil layer was significantly higher for NT-RT and NT-CT than that for NT-NT at the 5% level, but it was not significant in deeper soil during late rice harvest. The situ soil saturated hydraulic conductivity at 0～5 cm soil layer was significantly higher under NT-CT than those under NT-NT and NT-RT at the 5% level, but the difference was not significant at 5～10 cm soil layer during drying field periods for both early rice and late rice. Yields from the highest to the lowest were NT-RT, NT-CT, NT-NT in order for both early rice and late rice, and the key of yield components was effective panicles of rice.