Abstract: By 32P-labelled technique, five comparements with air gap was designed to examine time-course underground 32P transfer via VAM hyphae links between roots of red clover and its effects on host red clover growth. Plants were harvested at 6 weeks, 8 weeks and to weeks after planting. The results demonstrated that 32P can transfer between roots of red clover via VAM hyphae link and the total amount of 32P transfer didn't show significance difference at different harvest times. Shoot dry weight of receiver red clover increased at the third harvest time due to the formation of VAM hyphae linksbetween roots of donor and receiver plants. The factors which affected the 32P transfer via VAM hyphae were also discussed.