| 摘要: |
| 利用PCR技术从雄性中国荷斯坦牛的基因组DNA中克隆了Y染色体性别决定基因（Sex-determining Region on the Y Chromosome，SRY）的编码区全长序列，构建了表达载体pET-28a/SRY，并将其在大肠杆菌（E.coli）中进行了诱导表达，对表达产物进行了检测。结果表明，SRY基因编码区长687 bp，编码229个氨基酸；表达载体pET-28a/SRY构建成功；表达产物中含有相对分子质量为33ku的SRY蛋白。 |
| 关键词: 奶牛 Y染色体 SRY基因 SRY蛋白 诱导表达 |
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| Cloning of Holstein cow's SRY ORF and its expression in E.coli |
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| Abstract: |
| The gene of SRY ORF was cloned using Holstein cow genome.The total coding region is 687 bp,encoding a peptide with 229 amino acid residues.The Holstein cow’s SRY ORF was cloned to EcoRⅠ and SalⅠ sites of pET-28a(+) to construct an expression plasmid pET-28a/SRY.The expression plasmid was transformed to E.coli BL21 (DE3),induced by IPTG.SDS-PAGE analysis and confirmed that the Holstein cow’s SRY peptide was highly expressed at 4 h induction at 37 ℃.The SRY protein was expressed from totle protein of E.coli BL21 (DE3). |
| Key words: cow Y chromosome SRY gene SRY protein induced expression |
